Cloning into first hairpin site: 1 μl - 10 ng GFP Forward primer HP1 1 μl - 100 ng 5' primer HP1 1 μl - 100 ng 3' primer HP1 1 μl dNTPs (10 mM) 5 μl 10x Pfu reaction buffer 1 μl Pfu DNA Polymerase (Promega) 39 μl PCR-Grade water

OR

Cloning into second hairpin site: 1 μl - 10 ng LacZ Forward primer HP2 1 μl - 100 ng 5' primer HP2 1 μl - 100 ng 3' primer HP2 1 μl dNTPs (10 mM) 5 μl 10x Pfu reaction buffer 1 μl Pfu DNA Polymerase (Promega) 39 μl PCR-Grade water