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C6-volatile production from E. coli extracts expressing either a GST protein or a GST-94J16 fusion protein
| Extract | Volatile Production
|
|
|---|---|---|
| Hexanal | trans-2-Hexenal | |
| μg min−1 mg−1 protein | ||
| GST eluate | 5.3 ± 1.0 | UDa |
| GST-94J16 eluate | 6.1 ± 0.6 | 9.8 ± 0.1 |
Headspace volatiles were measured by GC after the addition of HPOD or HPOT substrate to affinity-purified protein eluate. Quantity of C6 volatiles was measured relative to known quantities of standard compounds.
UD, Undetectable.
