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Flow cytometry analysis gating strategies. (A) Forward scatter (FSC-H) and side scatter (SSC-H) characteristics of a PBMC population with the lymphocytes isolated in the quadrilateral gate. Gated-PBMCs labelled with both CD3-FITC and IL2-RPE, with (B) and without (C) prior stimulation with PMA, ionomycin and brefeldin.
