Figure 5. The number of PMNs and the concentration of 12-lipoxygenase products are increased in the BAL fluid in response to Pseudomonas aeruginosa intranasal infection.

Mice were challenged via intranasal inoculation with P. aeruginosa strain PAO1 (5 mice) or PBS (5 mice) and sacrificed after 18 hours. BAL fluid was collected and analyzed for cellular infiltrate, myeloperoxidase activity, and 12-lipoxygenase eicosanoids. The quantity of cellular infiltration (A) MPO activity (B) HXA₃ (C) and 12-HETE (D) present in the airspace after infection with PA01 was compared to PBS mock infection control and differences from control reaching statistical significance using a two-tailed unpaired Student’s T-test (p < 0.05) are represent by (*). In vivo experiments were performed on two separate occasions yielding similar results.