Figure 1.
Histological comparison of wild-type and Ercc1−/− tissue recombinants treated with T&E2 at 8 weeks post-renal grafting. (A) Prostate tissue from host Nude mouse. (B) Wild-type tissue recombinants displayed normal glandular architecture and focal hyperplasia. Ercc1−/− tissue recombinants displayed (C) 2+ PIN-like lesions, and (D) focal areas of nuclear pleomorphism, abnormal mitosis and crowding with epithelial proliferation. Arrows point to cells in mitoses. (A–D, scale bar = 50 μm) (E) Tissue sections were stained with Hoechst 33258 stain, with the nucleus of mouse origin having a spotty appearance while the cells of rat origin have a diffuse appearance. (left, scale bar = 100 μm; right, scale bar = 33 μm)