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. Author manuscript; available in PMC: 2013 Oct 24.
Published in final edited form as: J Am Chem Soc. 2012 Oct 15;134(42):17722–17730. doi: 10.1021/ja307859m

Table 3.

Activation parameters for kcat of WT and Y209W ecTSase.

TSase temperature range ΔH‡ kcal/mol TΔS‡ at 25 °C kcal/mol ΔG‡ kcal/mol
WT 5–35 °C 3.4 ± 0.2 −12.8 ± 0.2 16.2 ± 0.4
Y209W 5–25 °C 7.0 ± 0.3 −12.8 ± 0.4 19.8 ± 0.7
25–35 °C lower limit: ca. 30 lower limit: ca. 11 N.D.