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ζ-potential (a) and apparent hydrodynamic diameter (b) of PXT nanoparticles in the process of shell assembly. Shell: non-PEGylated (PLL/Hep)4 (1), and PEGylated (PLB16-5/Hep)7.5 (2), (PLB16-5/Hep)5/(PLB16-5/Hep)2.5 (3)