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. 2012 Sep 4;47(11):1334–1343. doi: 10.3109/00365521.2012.708940

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Copyright: © Informa Healthcare

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Figure 3. — Immunocytochemical staining of isolated human enterocytes for epithelial markers. (A)Top panel: isolated and cultured human EpCAM cells stained positive for cytokeratins 18, 20 and on activation with IFN-γ also expressed HLA-DR. Bottom panel: enterocyte-specific markers such as maltase glucoamylase and sucrase isomaltase were also found to be expressed by the cells. Staining for mucins showed the presence of few secretory epithelial cells – goblet cells which stained pink/magenta, or blue indicating the presence of neutral and acidic mucins. (B) Immunofluorescence staining of human enterocytes with antibodies to Toll-like receptors (TLRs) showed that these cells expressed TLR-6, -7, -8 and 10, thus confirming our FACS results. Magnification 20×, neutral mucin and acidic mucin 60×.