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. 2012 Sep 4;47(11):1334–1343. doi: 10.3109/00365521.2012.708940

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Copyright: © Informa Healthcare

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Figure 4. — Immunofluorescence staining of normal human small bowel tissue sections with antibodies to various epithelial markers. Indirect immunofluorescence single staining was performed for detection of CK8, CK18, CK19 and CK20. Positive staining for CK8, CK18 and CK19 was observed along the whole epithelial layer of crypt–villus axis while CK20-positive staining was observed only at the differentiated villus cells. Indirect immunofluorescence double staining for EpCAM (green) and enterocyte markers, sucrase isomaltase and maltase glucoamylase (both red) in small intestine was performed. EpCAM-positive reaction is found on basolateral and basal side of epithelial cells while sucrase isomaltase and maltase glucoamylase-positive reaction is observed on luminal side only. Positive reaction for sucrase isomaltase is present in crypt as well as villus cells but for maltase glucoamylase it is observed only in villus cells. Magnification 40×.