Table I.
Stimulation of pure rGAD1 or rGAD2 at pH 7.3
| Treatment | rGAD1
|
rGAD2
|
||
|---|---|---|---|---|
| GAD activity | Stimulation | GAD activity | Stimulation | |
| μmol CO2 min−1 mg−1 protein | -fold | μmol CO2 min−1 mg−1 protein | -fold | |
| −Ca2+/CaM (control) | 1.95 ± 0.4 | — | 1.9 ± 0.4 | — |
| +Ca2+ | 2.0 ± 0.6 | 0 | 5.6 ± 0.8 | 1.9 |
| +CaM | 5.5 ± 1.6 | 1.8 | 5.5 ± 0.9 | 1.9 |
| +Ca2+/CaM | 71.0 ± 7.1 | 35.4 | 26.9 ± 3.4 | 13.2 |
| +Ca2+/CaM/TFP | 32.0 ± 4.0 | 15.4 | 5.2 ± 1.2 | 1.7 |
rGAD1 and rGAD2 were assayed in 100 mm 1,3-bis-Tris-propane-HCl, pH 7.3, 10 mm NaCl, 100 μm PLP, and 20 mm glutamate ([1-C14]l-glutamate 0.02 μCi/mmol). When indicated, CaCl2 and CaM were added to final concentrations of 1 mm and 0.4 μm, respectively. The values are the averages ± se of two separate experiments.