Table IV. Largest flux sensitivities in bna572 for biomass tradeoff (oil, protein), as judged by slopes in flux magnitude of the lower and upper bounds at standard biomass composition (37.2% [w/w] oil).

Top 10 rankings are given for TAG and protein responsiveness. All sensitivities are in Supplemental Table S7. Subsystems are taken from Supplemental Table S1. N/A, Not applicable.

Rank	Reaction No.	Name of Reaction (Abbreviation); Subsystem	Lower Bound	Upper Bound
			nmol h−1 (% increase TAG)−1
Top oil-responsive reactions
1	22	Pyruvate kinase (PK_c); glycolysis	0.0	33.6
	26	Phosphofructokinase (PFK_c); glycolysis	0.0	33.6
	62	Fru-bisP aldolase (ALD_p); Calvin cycle; glycolysis	33.6	0.0
	63	Phosphofructokinase (PFK_p); glycolysis	0.0	33.6
2	27	Pyrophosphate-dependent phosphofructokinase (PFP_c); glycolysis	31.1	2.5
	25	Fru-bisP aldolase (ALD_c); glycolysis	2.5	31.1
3	19	Adenylate kinase (ADK_c); nucleotide metabolism	26.7	6.9
4	35	Malate dehydrogenase (MDH_m); citric acid cycle; malate-Asp shuttle	N/A	24.0
5	13	Phosphopyruvate carboxykinase (PPCK_c); glycolysis; PEP carboxylation	23.79	−1.32
6	21	Pyruvate, phosphate dikinase (PPDK_c); glycolysis	22.0	11.6
7	480–482	Pyruvate dehydrogenase (PDH_p); glycolysis	20.2	20.2
8	102	CO2 transport (c, p); transporter, symporter, c_p	N/A	19.7
9	44	Acetyl-CoA carboxylase (ACCase_p); fatty acid synthesis	18.5	18.5
10	58	Pyruvate kinase (PK_p); glycolysis	0	17.9
	49	Malate dehydrogenase (oxaloacetate-decarboxylating; NADP+; ME_p); carboxylic acid metabolism	0	17.9
	95	Pyruvate transport (c, p); transporter, symporter, c_p	0	17.9
Top protein-responsive reactions
1	88	Adenylate translocator (c, m); transporter, c_m, is_m	−20.2	−20.2
	91	H+-transporting two-sector ATPase (m); oxidative phosphorylation; transporter, is_m	−20.2	−20.2
	93	Phosphate carrier (c, m); transporter, symporter, c_m	−20.2	−15.6
2	90	NADH dehydrogenase (ubiquinone); oxidative phosphorylation; transporter, is_m	−8.7	−8.7
	463	Ubiquinol-cytochrome c reductase; oxidative phosphorylation; transporter, is_m	−8.7	−8.7
	464	Cytochrome c oxidase; oxidative phosphorylation; transporter, is_m	−8.7	−8.7
3	258	Storage protein synthesis	−6.6	−6.6
4	115 (116)	Gln exchange (ap, c); exchange	−4.7	−4.7
5	465	Oxygen transport (c, m); exchange	−4.4	−4.4
6	75	Gln/Glu antiport (Gln_c:Glu_p_anti); transporter, antiporter, c_p	−0.6	−3.6
7	79	Adenylate translocator (c, p); transporter, antiporter, c_p	−3.3	N/A
	106	Phosphate transport (c, p); transporter, uniporter, c_p	N/A	−3.3
	46	Glu dehydrogenase (GDH_p); ammonium assimilation	−3.3	−0.2
8	9	Glutaminase (c); Gln catabolism; deamination	0	−3
8	45	Glu synthase (NADPH; p); photorespiration; ammonium assimilation; Glu synthesis	0	−3
9	101	NH3 transport (c, p); transporter, uniporter, c_p	−0.2	−2.8
10	76	Glu/Mal antiport (c, p); transporter, uniporter, c_p	−2.6	−2.6