Table II. Comparison of the active site diameters of both PpAOC isoforms as measured across the β-barrel between amino acids forming the inner part of the binding cavity.
Distances were measured between Cα positions of PpAOCs Glu-18, PpAOC1 Leu-142 (PpAOC2 Leu-141), and PpAOC1 Leu-133 (PpAOC2 Leu-132) as well as PpAOC1 Tyr-91 (PpAOC2 Tyr-90), PpAOC1 Tyr-107 (PpAOC2 Tyr-106), PpAOC1 Ala-89 (PpAOC2 Ala-88), and PpAOC1 Cys-73 (PpAOC2 Cys-72). The distances were measured for all monomers of both apo and 12,13-EOD complexes of PpAOC1 and PpAOC2, and their averaged values with accompanying sd values are presented. The row on the right side presents the diameter difference between PpAOC2 and PpAOC1.
| PpAOC1 |
PpAOC2 |
||||
|---|---|---|---|---|---|
| Parameter | Average | sd | Average | sd | ifference |
| Å | |||||
| E18-Y91/Y90 | 12.42 | 0.032 | 12.60 | 0.054 | 0.179 |
| E18-Y107/Y106 | 13.55 | 0.060 | 13.70 | 0.071 | 0.140 |
| E18-C73/C72 | 15.50 | 0.057 | 15.44 | 0.082 | −0.055 |
| E18-A89/A88 | 14.88 | 0.085 | 15.09 | 0.071 | 0.208 |
| L142/L141-Y91/Y90 | 14.05 | 0.067 | 14.46 | 0.061 | 0.408 |
| L142/L141-Y107/Y106 | 10.94 | 0.071 | 11.33 | 0.122 | 0.380 |
| L142/L141-C73/C72 | 15.16 | 0.090 | 15.32 | 0.105 | 0.157 |
| L142/L141-A89/A88 | 14.32 | 0.057 | 14.85 | 0.099 | 0.53 |
| L133/L132-Y91/Y90 | 12.58 | 0.055 | 12.90 | 0.070 | 0.321 |
| L133/L132-Y107/Y106 | 7.65 | 0.045 | 8.13 | 0.126 | 0.480 |
| L133/L132-C73/C72 | 14.18 | 0.078 | 14.33 | 0.082 | 0.153 |
| L133/L132-A89/A88 | 11.51 | 0.065 | 12.03 | 0.089 | 0.522 |