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. 2012 Sep 19;160(3):1267–1280. doi: 10.1104/pp.112.204180

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Figure 8. — Subcellular localization of H. calycinum CNL in N. benthamiana leaf epidermis cells. A and B, Transient expression of YFP-CNL (A) and CNL-DEL-YFP (B) constructs resulted in the formation of peroxisomal and cytosolic CNL proteins, respectively. C to F, Cotransformation with YFP-CNL (C) and the marker construct CFP-PTS1 (D) led to overlayable white fluorescence in peroxisomes (F), which did not match the autofluorescence of chloroplasts (E). G and H, Fluorescence intensities of YFP and CFP in three peroxisomes marked by a white arrow (G) were recorded (H). J, Emission fingerprints of CFP (*), YFP (**), and chlorophyll (***) are documented. Bars = 20 µm.