Table I. Product analysis by LC-ESI-MS.

CNL assays containing substrates that caused unexpected enzyme activity in the luciferase-based assay and are marked by asterisks in Figure 5A were analyzed for CoA ester formation by LC-ESI-MS (negative ion mode). The results are presented as percentages of cinnamoyl-CoA formation, which was set as 100%. ND, Not detectable.

Substrate	CoA Ester Formation
Cinnamic acid	100
4-Hydroxybenzoic acid	ND
3-Hydroxybenzoic acid	ND
2-Hydroxybenzoic acid	ND
Vanillic acid	ND
Gallic acid	<0.01% of cinnamoyl-CoA formation
Shikimic acid	ND
Mandelic acid	ND
Malic acid	<0.05% of cinnamoyl-CoA formation
l-Phe	ND