Table III. Primers used.
Primer | Sequence |
---|---|
1 | 5′-ATGGATAAGCTCCCAAAATGTGGTGCC-3′ |
2 | 5′-TTAGCCATATCCCTCAACTGGGCC-3′ |
3 | 5′-TCAGCAGCGTGGAGGTCGAGTCG-3′ |
4 | 5′-TTAAAGACGAGACATGGCAAG-3′ |
5 | 5′-ATTGAATTCTTAAAGACGAGACATGGCAAG-3′a |
6 | 5′-CTGCATTCCTTGATCTTGTTC-3′ |
7 | 5′-ACATCGAGGAGAATCTGAAGAG-3′ |
8 | 5′-TGATGGTATCTACTACTCGG-3′ |
9 | 5′-AATATACGCTATTGGAGCTGG-3′ |
10 | 5′-GGGGACAAGTTTGTACAAAAAAGCAGGCTTAACCATGGATAAGCTCCCAAAATGTGG-3′ |
11 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCTTAAAGACGAGACATGGCAAGAAC-3′ |
12 | 5′-GGGGACCACTTTGTACAAGAAAGCTGGGTCAAGACGAGACATGGCAAGAACAGG-3′ |
EcoRI restriction site is underlined.