Figure 1.

Structure of the CCD8 gene family in grasses. A, Phylogenetic relationships of grass species with completely sequenced genomes. Number of CCD8 gene copies per genome is in parentheses. B, List of CCD8 genes with number of exons in each homolog. C, Phylogenetic relationships of protein sequences for genes in B (left) and a schematic summary of local-order synteny for genes in the vicinity of CCD8 homologs (right). Gene phylogeny was determined with Clustal and rooted with Arabidopsis MAX4. Nodes depicted with thick lines have bootstrap values >90%. The clade of putative D10 orthologs is highlighted by a shaded rectangle enclosed by a dashed line. Data are adapted from the Phytozome synteny database at www.phytozome.net. The CCD8 homologs are shown as filled boxes positioned at the center of each graphic, along with the nearest five upstream and downstream neighboring genes. Numbers in boxes indicate families of homologous genes. Putative orthologs of rice D10 are shaded dark, CCD8 paralogs shaded light, and genes lacking synteny with other genomes in gray unnumbered boxes. Where sets of tandem CCD8 gene copies occur in the foxtail millet genome (Si000867 and Si004869), these are respectively labeled with single (‘) and double (“) quotation marks. [See online article for color version of this figure.]