Table III. Differential expression of putative Brachypodium kinases altered in PMV and PMV+SPMV infection.
The relative unlogged fold change (≥2-fold) in gene expression of PMV and PMV+SPMV infection compared with mock are indicated. Minus sign represents down-regulated fold-change. The gene descriptions were deduced from Pfam and PANTHER functional annotations obtained using the Phytozome (Goodstein et al., 2012). The statistical significance of gene expression changes was determined using Student’s t test and was corrected for FDR using the Benjamini and Hochberg method (q-value < 0.05).
| Gene ID | PMV | PMV+SPMV | Description |
|---|---|---|---|
| Bradi4g02020 | 5.15 | 5.36 | LRR protein kinase, RLK plant type, subfamily LRR-VIII-2 |
| Bradi1g32630 | 2.25 | 2.10 | Protein kinase domain, RLK plant type, subfamily LRR-VIII-2 |
| Bradi4g11740 | 23.97 | 21.17 | LRR N-terminal domain, RLK plant type, subfamily LRR-XII |
| Bradi5g21857 | 2.91 | 3.02 | LRR, Di-Glc binding within endoplasmic reticulum, RLK plant type |
| Bradi4g02850 | 5.19 | 6.07 | WAK, RLK plant type, subfamily WAK/LRK10L-1 |
| Bradi1g02210 | 6.88 | 8.33 | Calcium-binding EGF domain, WAK, RLK plant type |
| Bradi3g01157 | 2.66 | 2.55 | Calcium-binding EGF domain, WAK, RLK plant type |
| Bradi4g21990 | 4.05 | 4.53 | Protein kinase domain, RLK plant type, subfamily RLCK-OS2 |
| Bradi1g75140 | 2.40 | 2.31 | Protein kinase domain, RLK plant type, subfamily RLCK-IV |
| Bradi4g38690 | 3.68 | 4.76 | U-box domain, protein kinase domain, RLK plant type, subfamily RLCK-IXb |
| Bradi4g37220 | 5.72 | 5.36 | d-Man binding lectin, S-locus glycoprotein, RLK plant type, subfamily SD-1c |
| Bradi5g02980 | 3.51 | 2.82 | d-Man binding lectin, RLK plant type, subfamily SD-2b |
| Bradi2g20687 | 2.37 | 3.00 | d-Man binding lectin, S-locus glycoprotein, RLK plant type |
| Bradi4g24590 | 3.20 | 4.19 | Protein kinase domain, RLK plant type |
| Bradi2g47510 | −5.26 | −7.14 | MAPKK related, subfamily MEKK_ste11_MAP3K |
| Bradi4g30380 | −3.23 | −3.33 | NAF domain, calcium/calmodulin-dependent protein kinase, subfamily KIN1/SNF1/Nim1_like (CAMK_2) |
| Bradi4g45310 | −2.22 | −2.78 | AGC kinases, PAS fold, subfamily PVPK_like (AGC_8)_kin82y |
| Bradi1g57700 | 6.33 | 6.94 | Protein kinase domain, RLK plant type, subfamily l-LEC |
| Bradi1g23710 | 5.64 | 4.92 | Legume lectin domain, RLK plant type, subfamily l-LEC |
| Bradi1g23730 | 11.32 | 9.39 | Legume lectin domain, RLK plant type, subfamily l-LEC |
| Bradi1g57657 | 5.45 | 7.01 | Legume lectin domain, RLK plant type, subfamily l-LEC |
| Bradi1g57690 | 3.38 | 4.23 | Legume lectin domain, RLK plant type, subfamily l-LEC |
| Bradi5g16760 | 3.37 | 3.17 | Legume lectin domain, RLK plant type, subfamily l-LEC |
| Bradi1g08990 | −2.38 | −2.00 | Protein kinase domain, subfamily l-LEC |
| Bradi1g25647 | 2.32 | 2.56 | Domain of unknown function DUF26, RLK plant type |
| Bradi1g42397 | −2.04 | −2.13 | Domain of unknown function DUF26, RLK plant type |
| Bradi1g25717 | 2.54 | 2.26 | Domain of unknown function DUF26, RLK plant type |