Figure 3.
Targeting of Vac8p to the yeast vacuolar membrane in yeast mutant cells by Arabidopsis PATs. Vac8p.GFP is targeted to the vacuolar membrane in wild-type cells (Wt; indicated on the right). In the 5xΔ strain (indicated on the right) containing the empty plasmid as a control (vector, indicated on the left), Vac8p.GFP associates with ER structures. Expression of AtPATs (indicated on the left) can retarget Vac8p.GFP back to the vacuolar membrane, while the PAT21CA mutant is not able to retarget Vac8p. First row shows a bright-field picture, and the GFP fluorescence is depicted in the second row. FM4-64 staining of the yeast vacuole is shown in the third row, which was merged with the GFP fluorescence picture (shown in the last row). Bars = 10 µm. [See online article for color version of this figure.]