Table II.
Enzymatic activity of wild-type and mutant CapTKT1 and CapTKT2
| Enzyme | Activity |
|---|---|
| % | |
| Wild-type CapTKT1 | 100 |
| CapTKT1 (Glu-491 to Ala) | 0.8 |
| Wild-type CapTKT2 | 100 |
| CapTKT2 (Glu-449 to Ala) | 0.1 |
Mono-Q-purified wild-type and mutant CapTKT1 and CapTKT2 were used for the different assays. CapTKT1 activity was determined using d-Rib-5-phosphate and d-xylulose-5-phosphate as substrates. CapTKT2 activity was determined using d-glyceraldehyde-3-phosphate and [2-14C]pyruvate as substrates. The indicated Glu residue was mutated into Ala as shown by the arrow.