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. 2012 Sep 13;160(3):1567–1580. doi: 10.1104/pp.112.205799

Figure 7.

Figure 7.

Transcript levels for select GA metabolism genes in Arabidopsis seedlings. The levels of transcript for KS, GA2ox2, GA3ox1, GA20ox1, GA20ox2, and GA20ox3 relative to ACT2 in ebf2-3 and ein3-1 eil1-1 mutants were normalized to levels in untreated Col seedlings. Seedlings were grown in the dark in air for 2 d, RNA was extracted, and the transcript was analyzed with qRT-PCR. Transcript levels in untreated seedlings (closed bars) were compared with seedlings treated with 1 µL L−1 ethylene for 2 h before RNA extraction (open bars). Data shows the average ± sem for three biological replicates with two to three technical replicates each. aStatistical difference compared with untreated Col (P ≤ 0.05); bstatistical difference caused by treatment with ethylene compared with untreated seedlings of that background (P ≤ 0.05); cstatistical difference caused by ethylene compared with ethylene-treated Col seedlings (P ≤ 0.05).