Table V. Validation of the Glc-deprivation response of the detached dark-held inflorescences.
Detached Landsberg erecta inflorescences were held in water in the dark for 24 h, in water supplemented with 3% Glc in the dark for 24 h, or in water in the light for 24 h. P value indicates moderated Student's t test results for significance of the microarray change; MA indicates fold change in transcript abundance in microarray. *24 h Dark and 24 h Dark + Glc are significantly different (based on lsd test); +24 h Dark + Glc and 24 h Light are significantly different (based on lsd test). Data are means of pooled five to six inflorescences from three independent biological experiments. Measurements were normalized to ACTIN2. The results from the individual qRT-PCR experiments and how the transcript abundance of each gene changes in aging leaves are presented in Supplemental Table S13.
| Name | Arabidopsis Genome Initiative No. | P Value | MA | 24 h Dark | 24 h Dark + Glc | 24 h Light |
|---|---|---|---|---|---|---|
| Reference | ||||||
| ACT2/actin2 | At3g18780 | 0.40 | 1.34 | 1.00 | 1.00 | 1.00 |
| SKP1/E3 ubiquitin ligase | At1g75950 | 0.79 | 1.14 | 1.66 | 1.15 | 1.12 |
| UBQ14/ubiquitin | At4g02890 | 0.58 | 0.82 | 1.45 | 1.16 | 0.84 |
| Up-regulated | ||||||
| ANAC029/ATNAP/transcription factor | At1g69490 | 0.06 | 90.46 | 45.93 | 15.29* | 9.43 |
| ANAC092/ORE1/transcription factor | At5g39610 | 0.03 | 29.62 | 27.90 | 13.76* | 5.01 |
| ERF/RAP2.3/ethylene response transcription factor | At3g16770 | 0.03 | 7.59 | 26.99 | 11.42* | 5.10 |
| GBF1/G-box-binding transcription factor | At4g36730 | 0.03 | 10.82 | 10.14 | 4.34* | 1.55+ |
| RPK1/Leu-rich receptor-like kinase | At1g69270 | 0.04 | 4.93 | 4.81 | 2.70* | 1.77 |
| SEN4/xyloglucan endotransglucosylase | At4g30270 | 0.08 | 19.20 | 10.70 | 5.09* | 4.90 |
| SAG12/Cys protease | At5g45890 | 0.75 | 0.91 | 5.56 | 24.56 | 54.55 |
| ATG18G/autophagy18G | At1g03380 | 0.04 | 4.52 | 5.15 | 3.76 | 1.40 |
| ATG8E/autophagy 8E | At2g45170 | 0.04 | 17.92 | 14.77 | 5.66* | 3.33 |
| ATG13/autophagy 13 | At3g49590 | 0.03 | 5.62 | 8.11 | 4.07 | 2.36 |
| GDH2, Glu dehydrogenase | At5g07440 | 0.03 | 8.51 | 7.01 | 2.56* | 1.37 |
| PIF5/PIL6/phytochrome-interacting factor 5 | At3g59060 | 0.03 | 8.61 | 5.93 | 2.86* | 1.79 |
| PIF4/phytochrome-interacting factor 4 | At2g43010 | 0.03 | 3.31 | 6.69 | 2.85 | 1.38 |
| Down-regulated | ||||||
| NDF4/subunit of the chloroplast NAD(P)H dehydrogenase complex | At3g16250 | 0.03 | 0.06 | 0.05 | 0.13 | 0.72+ |
| CPN60B/chaperonin 60B | At1g55490 | 0.03 | 0.21 | 0.24 | 0.37 | 0.42 |
| CPN60A/chaperonin 60A | At2g28000 | 0.03 | 0.16 | 0.29 | 0.40* | 0.33 |
| GAPB/glyceraldehyde-3-phosphate dehydrogenase B subunit | At1g42970 | 0.03 | 0.12 | 0.16 | 0.20 | 0.97+ |
| GAPA-2/glyceraldehyde-3-phosphate dehydrogenase A subunit 2 | At1g12900 | 0.03 | 0.38 | 0.26 | 0.28 | 0.54 |
| Phosphoglycerate kinase | At1g56190 | 0.03 | 0.19 | 0.28 | 0.36 | 0.78+ |
| SBPASE/sedoheptulose-bisphosphatase | At3g55800 | 0.03 | 0.16 | 0.15 | 0.20 | 1.05+ |
| Transketolase | At3g60750 | 0.03 | 0.29 | 0.38 | 0.42 | 0.91+ |
| ATFBA5/Fru-bisP aldolase 5 | At4g26530 | 0.03 | 0.04 | 0.11 | 0.13 | 2.88+ |
| ATFBA2/Fru-bisP aldolase 2 | At4g38970 | 0.03 | 0.21 | 0.24 | 0.22 | 0.80+ |
| GLDP1/Gly decarboxylase | At4g33010 | 0.03 | 0.11 | 0.14 | 0.13 | 1.67+ |
| SHM1/Ser hydroxymethyltransferase 1 | At4g37930 | 0.03 | 0.21 | 0.27 | 0.22 | 1.85+ |
| FD-GOGAT/Glu synthase | At5g04140 | 0.03 | 0.24 | 0.32 | 0.29 | 1.55+ |
| DCT/dicarboxylate transport 2.1 | At5g64290 | 0.04 | 0.30 | 0.52 | 0.48 | 0.64+ |
| ATCLH1/CORI1/chlorophyllase | At1g19670 | 0.04 | 0.11 | 0.19 | 0.31 | 0.71+ |