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. 2012 Aug 6;9:149. doi: 10.1186/1743-422X-9-149

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Copyright ©2012 Mukherjee et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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TUNEL assay analysis of Apoptosis in control and NS1 transfected cells. The cells were analyzed 24 h post transfection using fluorescently labeled anti-BrdU antibodies. The fluorescence was detected microscopically. Left panel are cells visualized under visible light without filter; Right panel are cells visualized under specific filters for fluorescence detection. The left and the right panel are the cells under same microscopic field A. Untransfected control cells B. Cells transfected with pcDNA 3.0 (-) vector C. cells transfected with pcDNA3-H5N1-NS1 construct D. cells transfected with pcDNA3-H11N1-NS1 construct.