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. 2012 Aug 6;9:149. doi: 10.1186/1743-422X-9-149

Table 2.

Analysis of percentage of apoptotic cells in control, mock transfected and NS1 transfected cells as observed in TUNEL assay experiment

Constructs Percentage (%) of positive cells per field out of 106cells used in TUNEL assay
Control
 0
Vector (pcDNA3.0)
 0
H5N1-NS1
 40%
H11N1-NS1 20%

The cells were analyzed 24 h post transfection using fluorescently labeled anti-BrdU antibodies. The fluorescence was detected microscopically. The percentage of fluorescent cells is represented as the degree of apoptosis in transfected cells.