Table 1.
Key vectors used in this study
| Vector properties | ||||||
|---|---|---|---|---|---|---|
|
Vector |
Gene 1 |
Gene 2 |
Cloning sites (Gene1) |
Cloning sites (Gene2) |
Replicon |
Antibiotic resistance |
| pHGGWA |
HisGST- CYCD3;1 |
|
Gateway |
|
ColE1 |
Ampicillin |
| pHMGWA |
HisMBP-SDS |
|
Gateway |
|
ColE1 |
Ampicillin |
| |
HisMBP-CYCB1;2 |
|
Gateway |
|
ColE1 |
Ampicillin |
| pHGGWA |
HisGST- Wee1 |
|
Gateway |
|
ColE1 |
Ampicillin |
| |
HisGST- CDKF;1 |
|
Gateway |
|
ColE1 |
Ampicillin |
| pCDFDuet-1 |
StrepIII- CDKA;1 |
GST-Cak1 |
NcoI - NotI |
NdeI - XhoI |
CloDF13 |
Spectinomycin |
| |
StrepIII- CDKB1;1 |
GST-Cak1 |
NcoI - NotI |
NdeI - XhoI |
CloDF13 |
Spectinomycin |
| |
StrepIII- CDKB2;2 |
GST-Cak1 |
NcoI - NotI (SLIC†) |
NdeI - XhoI |
CloDF13 |
Spectinomycin |
| |
|
GST-Cak1 |
|
NdeI - XhoI |
CloDF13 |
Spectinomycin |
| |
StrepIII- CDKA;1 |
|
NcoI - NotI |
|
CloDF13 |
Spectinomycin |
| |
|
GST-CDKF;1 |
|
NdeI - XhoI |
CloDF13 |
Spectinomycin |
| StrepIII- CDKA;1 | GST-CDKF;1 | NcoI - NotI | NdeI - XhoI | CloDF13 | Spectinomycin | |
†sequence and ligation-independent cloning.