Figure 3.

RNA gel-blot analysis of relative expression levels of the synthetic and wild-type B.t.-toxin genes in plant cells. Accumulation of synthetic (SYN) and wild-type (WT) B.t.-toxin mRNAs was compared in transiently transformed maize and tobacco cells and in stably transformed tobacco cells and Arabidopsis plants. A, Plasmids containing wild-type or synthetic B.t.-toxin genes were electroporated into maize BMS and tobacco BY-2 protoplasts. Plasmids containing a human β-globin gene were coelectroporated in both types of experiments to serve as an internal standard. The positions of the B.t.-toxin and globin transcripts are indicated. B, Tobacco BY-2 cells and Arabidopsis plants were stably transformed with constructs containing wild-type or synthetic B.t.-toxin genes and a GUS gene serving as an internal standard. The positions of the B.t.-toxin and GUS transcripts are indicated.