Figure 8.

The tumor-suppressive effects of MKRN1 knockdown via stabilization of p14ARF were observed in the p53 mutated gastric cancer cell line SNU601. A–C) Mice (N = 4) were inoculated subcutaneously in both flanks with 10⁶ cells of each SNU601 cell line. The stable SNU601 cell lines were prepared in the same ways as mentioned in Figure 7. A) At 23 days after inoculation, mice were killed in 7.5% CO₂ chamber, and mice and excised tumors from mice were photographed. B) Quantification of tumor formation was performed by measurement of tumor size 4, 6, 8, 11, and 13 days after inoculation (N = 4 tumors for four mice). The error bars indicate 95% confidence intervals. *, P = .02; **, P = .01, two-sided t test. All statistical tests were two-sided. C) Immunohistochemical analysis and hematoxylin and eosin (H&E) staining were performed for each group of tumors as described in Materials and Methods (magnification ×200). SA-β-galactosidase-stained slides were viewed under ×200 magnification. The scale bar indicates 100 µm. MKRN1 = Makorin ring finger protein 1; p14ARF = p14 alternative reading frame.