Skip to main content
. 2012 Nov 6;7(11):e48675. doi: 10.1371/journal.pone.0048675

Figure 4. Role of Ascl1 in the upregulation of genes of the Dlk1-Gtl2 locus in Ngn2 KO mice. A.

Figure 4

Upregulation of Ascl1 mRNA in the dorsal telencephalon from WT or Ngn2 KO was determined by ISH (E12.5, left panel) and qPCR experiments (E13.5, right panel). B. Expression of imprinted RNAs from the Gtl2-Dlk1 locus (Dlk1, Gtl2, Rtl1, Mirg, Rian) in WT, Ngn2 KO, Ascl1 KO and Ngn2/Ascl1 double KO in E13.5 dorsal telencephalons. Data are presented as percentage of change normalized to the mean of WT (taken as 100%) + s.e.m; *P<0.01 in Mann-Whitney’s test. C. RNA expression (ISH) of Gtl2 in WT and Ascl1 KO mice at E12.5 and E14.5. D. RNA expression (ISH) of DLK1 in WT and Ascl1 KO mice at E13.5. Red arrows in C and D indicate regions in the ventral telencephalon where expression of Gtl2 and DLK1 are reduced in Ascl1 KO mice. Scale bars: A,B,D = 200 µm, C = 400 µm.