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. 2012 Nov 6;7(11):e48611. doi: 10.1371/journal.pone.0048611

Figure 3. Inhibition of JNK activity reduces glucose-stimulated ADAR2 expression and auto-editing in β-cells.

Figure 3

INS-1 cells maintained at 2.8 mM glucose for 12 hours were cultured without serum for 6 hours at 2.8 mM glucose before pretreatment for 30 min with DMSO (−) or 10 or 20 µM JNK inhibitor SP600125 (JNKi). Cells were then cultured for 3 or 24 hours at 2.8 or 16.7 mM glucose in the presence of DMSO or JNKi at 10 or 20 µM as indicated. (A) The relative mRNA abundance of Adar2 was determined by quantitative RT-PCR. Data represent the mean±SEM from three independent experiments. *P<0.05, **P<0.01. (B) Lysates from cells treated for 24 hours were analyzed by immunoblotting using the indicated antibodies. Results are representative of three independent experiments. (C) The efficiency of ADAR2 auto-editing was analyzed by RT-PCR for cells treated for 24 hours. Densitometric quantifications are shown as the mean±SEM (n = 3 independent experiments). *P<0.05, **P<0.01.