Table 1.
C. defragrans strains |
Geranic acid concentration [μM] |
|
---|---|---|
α-Phellandrene | β-Myrcene | |
65Phen (wild type) |
0.24 ± 0.01 |
8.85 ± 0.6 |
Δldi |
n.d. |
n.d. |
Δldicomp |
0.33 ± 0.24 |
6.61 ± 0.19 |
ΔgeoA |
n.d. |
4.96 ± 1.58 |
ΔgeoAcomp | 0.89 ± 0.25 | 11.79 ± 0.31 |
C. defragrans cultures were grown in 150 mL with 6 mM α-phellandrene or β-myrcene and 10 mM nitrate at 30°C and 130 rpm. Inoculum size was 1% (v/v). Duplicate determination. Detection limit for geranic acid was 6.4 nM. n.d. = not detectable.