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. 2012 Sep 4;12:192. doi: 10.1186/1471-2180-12-192

Table 2.

Physiological properties of C. defragrans strains growing with different monoterpenes

  α-Phellandrene
 Limonene
 β-Myrcene
65Phen ΔgeoA ΔgeoAcomp 65Phen ΔgeoA ΔgeoAcomp 65Phen ΔgeoA ΔgeoAcomp
MaxOD660
 0.321
 0.217
 0.342
 0.318
 0.174
 0.347
 0.155
 0.066
 0.149
Generation time [h]
 9.8
 34.9
 13.5
 25.4
 50.8
 44.9
 46.9
 57.1
 45.8
NO3- consumed [mM]
 10
 10
 10
 10
 10
 10
 7.3
 5.8
 8.1
NO2- formed [mM]
 0
 0
 0
 0
 0
 0.01
 0.22
 0
 0.009
Biomass formed [g/L] 0.34 0.23 0.32 0.35 0.22 0.35 0.14 0.08 0.17

C. defragrans strains 65Phen (wild type), ΔgeoA and ΔgeoAcomp were grown under standard conditions at 28°C for 280 h (α-phellandrene, limonene) or for 304 h (β-myrcene) with 4 mM monoterpene (in HMN) and 10 mM nitrate. As negative control served a culture without inoculum.