Table 3.
Strains and plasmids used in this study
| Strains or plasmids | Genotype, markers and further characteristics | Source/reference |
|---|---|---|
| Strains |
|
|
|
E. coli |
|
|
| S17-1 |
Thi, pro, hsdR, recA with RP4-2[Tc::Mu-Km::Tn7] |
[63] |
| One Shot®Top10 |
F- mcrA Δ(mrr-hsdRMS-mcrBC) φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ(araleu) 7697 galU galK rpsL (StrR) endA1 nupG |
Invitrogen |
|
C. defragrans |
|
|
| 65Phen |
Wild type |
[40] |
| 65Phen-RIFa |
RaR |
This study |
| 65Phen-RIF Δldib |
RaR, Δldi |
This study |
| 65Phen-RIF Δldicompc |
RaR, Δldi, pBBR1MCS-4ldi |
This study |
| 65Phen-RIF ΔgeoAd |
RaR, ΔgeoA |
This study |
| 65Phen-RIF ΔgeoAcompe |
RaR, ΔgeoA, pBBR1MCS-2geoA |
This study |
| Plasmids |
|
|
| pCR4-TOPO |
AmR, KmR, lacZα |
Invitrogen |
| pK19mobsacB |
KmR, sacB modified from B. subtilis, lacZα |
[64] |
| pK19mobsacBΔldi |
KmR, sacB modified from B. subtilis, lacZα, ORF25, ORF27 |
This study |
| pK19mobsacBΔgeoA |
KmR, sacB modified from B. subtilis, lacZα, ORF29-30, ORF32 |
This study |
| pBBR1MCS-4 |
AmR , mob, lacZα |
[65] |
| pBBR1MCS-4ldi |
AmR, mob, lacZα, ldi |
This study |
| pBBR1MCS-2 |
KmR, mob, lacZα |
[65] |
| pBBR1MCS-2geoA | KmR, mob, lacZα, geoA | This study |
a abbreviated in course of the text to C. defragrans RIF, b abbreviated to C. defragrans Δldi, c abbreviated to C. defragrans Δldicomp, d abbreviated to C. defragrans ΔgeoA, e abbreviated to C. defragrans ΔgeoAcomp.