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. 2012 Nov 6;7(11):e48765. doi: 10.1371/journal.pone.0048765

Figure 5. FLAG-tagged PcG proteins are bound to the en PRE in both the “ON” and “OFF” transcriptional states.

Figure 5

(A–D) X-ChIP (with α-FLAG) was performed on third instar imaginal discs and CNS, with en-GAL4 or ci-GAL4 driven Pho-FLAG (A), Sce-FLAG (B), Esc-FLAG (C), FLAG-Scm (D). Results are shown as a percentage of the input DNA, collected prior to ChIP. ns = not significant, * P≤0.05, ** P≤0.01, *** P≤0.001, **** P≤0.0001 (un-paired, two-tailed t-tests). Results shown are from three independent biological samples with 2 replicates each. (E) Fold increase (PRE/control) using the means from the experiments shown in A–D. The UAS-lines are shown on the left, with the drivers en-Gal4 (en) and ci-Gal4 (ci) on top.