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. 2012 Nov 6;7(11):e48795. doi: 10.1371/journal.pone.0048795

Figure 4. RANK expression levels under adherent and non-adherent conditions.

Figure 4

A . BMMs under the adherent condition were harvested (0 h) and subsequently cultured again under adherent and non-adherent conditions in the presence or absence of M-CSF (50 ng/ml), TGF-β (1 ng/ml), and RANKL (150 ng/ml) for the indicated periods. Relative expression levels of RANK mRNA were determined by real time RT-PCR. Data represent mean values of three independent experiments, with error bars indicating ± SD. **P< 0.01 for adherent condition vs. non-adherent condition at the same time point. B . Left panel: BMMs grown under the adherent condition were transferred to the non-adherent condition and further cultured for the indicated time periods. Right panel: BMMs under the non-adherent condition were transferred to the adherent condition and further cultured for the indicated time periods. RANK mRNA expression was analyzed by RT-PCR. C. Left panel: comparison of RANK protein expression levels between BMMs cultured under the adherent condition and those transferred to the non-adherent condition. Right panel: Comparison of RANK protein levels between BMMs cultured under the non-adherent condition and those transferred to the adherent condition. RANK protein expression on the surface of BMMs was analyzed by flowcytometry.