Figure 7. Differential extraction of Cah3 polypeptide from PSII core complexes isolated from C. reinhardtii cells grown under high-CO₂ (A) or acclimated to low CO₂ conditions for 4 h (B).

PSII hydrophobic and hydrophilic proteins were extracted from PSII core complexes using a chloroform/methanol (2∶1, v/v) mixture (see Materials and Methods). Immunoblot analysis of integral (I) and peripheral (P) protein fractions from PSII core complexes were probed with antibodies against Cah3 (Cah3) and PsbO protein (PsbO). The lanes were loaded with 10 µg protein.