Figure 2. Identification of cyslabdan-binding proteins.

Biotinylcyslabdan was attached to avidin beads and incubated with an MRSA lysate. After washing nonspecific proteins with PBS, the bound proteins were recovered from the beads via denaturation with Laemmli sample buffer and were then analyzed via SDS–PAGE. Typical data from five separate experiments are shown. The arrow indicates a band around 50 kDa that was detected reproducibly.