Table 1. Potential problems and solution during the analyses of HA-glycan interaction using SPR-based analyses.
| Step | Observation | Probable reason | Solution |
|---|---|---|---|
| Step 1 |
No increase of response |
Biotin capture reagent is inactivated or CAP chip is damaged |
Prepare new capture reagent and change the CAP chip |
| Step 2 |
No increase of response |
Glycan binding failed |
Re-inject the freshly prepared glycans |
| Step 3 |
No increase of response |
HA failed form the trimeric structure |
Use higher HA conc. (up to 1 μM) |
| Step 4 | The response should decrease | Regeneration is not complete | Repeat the regeneration step |