Figure 1. Sequence alignment of AcCYS to the other cystatins.
The sequence of AcCYS is compared with taro, rice, potato, human cystatin F, chicken egg white cystatin, human stefin A, and human stefin B. The characteristic motifs for putative interaction with target cysteine protease are marked by green asterisks. The unique motif, LARFAVxExN of phytocystatins is denoted by the red dots on top of the sequence. The α-helix and β-sheet regions are colored in red and blue, respectively.