NK-92 effector cells were transfected with mRNA (56% transgene expression, filled symbols) or lentiviral vector-transduced and sorted (> 95% transgene expression, open symbols). The expressed transgenes were GFP (circle), αCD19-CAR (square) or αCD20-CAR (triangle). Transgene expression did not negatively affect NK-92 killing of Raji, whereas SUP-B15 and TMD5 were resistant to non-CAR-expressing NK-92. αCD19-CAR-expressing NK-92 displayed higher cytotoxicity against TMD5 than αCD20-CAR. The data are representative of 2–5 assays. LV, lentiviral vector.