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. Author manuscript; available in PMC: 2013 Nov 15.
Published in final edited form as: J Neurosci Methods. 2012 Sep 6;211(2):179–184. doi: 10.1016/j.jneumeth.2012.08.024

Figure 3. Hematoxylin and Eosin stained spinal cord cross sections.

Figure 3

Selected spinal cord cross sections with H&E staining are shown here to reflect a normal thoracic cross-section caudal to lesion area of an injured rat (a); a spinal cord cross section with approximately 49% (close to goal hemisection injury) spared tissue remaining (b); and a spinal cord cross section with 38% (close to average) spared tissue remaining (c). Spared spinal cord tissue has been outlined to demonstrate full cross-section area (a) and distinguishing between spared and injured tissue (b & c).

For all sections (n=15), spared tissue remaining was 39.39% (16.44), average (standard deviation) Dorsal/ventral (D/V) and right/left (R/L) orientation and 500 um scale bar indicated for spinal cord cross sections, taken at 2.5x magnification