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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Med. 2012 Sep;18(9):1413–1417. doi: 10.1038/nm.2886

Figure 4.

Figure 4

Viral expression of GATA1 in rodent PFC causes depressive behavior. (a) Schematic diagram of the experimental schedule. (b) Representative low power (left panel) image of GFP immunohistochemistry (IHC) demonstrating the location of the viral infusion site in the medial PFC. The right panel shows the labeled neurons at higher magnification. (c,d) Influence of rAAV-control and rAAV-GATA1 on behavior in the forced swim test (FST, c) and learned helplessness (LH, d) paradigm. There was no effect on levels of locomotor activity (LA) (not shown). Results represent the mean ± S.E.M. (n = 9 for rAAV-control and n = 10 for rAAV-GATA1). * P < 0.05 compared to rAAV-control (t-test). (e) Schematic diagram of Gata1 shRNA knock down in the chronic unpredictable stress (CUS) paradigm. (f) Representative image of GFP immunohistochemistry demonstrating the location of viral infusion in the same region of the PFC as shown in b. (g) Influence of rAAV-ScrshRNA and rAAV-GATA1shRNA on sucrose preference in control and CUS exposed rats. Results represent the mean ± S.E.M. (Control: n = 6 for rAAV-ScrshRNA; n = 8 for rAAV-GATA1shRNA; CUS: n = 7 for rAAV-ScrshRNA; n = 8 for rAAV-GATA1shRNA). ** P < 0.05 compared to Control group (ANOVA and Fishers PLSD post-hoc analysis).