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. Author manuscript; available in PMC: 2013 Mar 1.
Published in final edited form as: Nat Med. 2012 Sep;18(9):1413–1417. doi: 10.1038/nm.2886

List of PCR primers used in this study for validation

mRNA expression
Gene Forward (5'->3') Reverse (3'->5')
CALM2 GGAATTAGTCCGAGTGGAGAG TGCTCTTCAGTCAGTTGGTCA
SYN1 AGATTTTTGGGGGACTGGAC TGACCACGAGCTCTACGATG
SYN3 CCAGATAGCTCCACCAGCTC GGATCCTGGAGAGGAGAAGG
SYNGR3 AGCTGCTCCTCTCTCTGTGG TAGGACAGGGCTGGTGTCTT
AMPH CCAACCATGAAGAGGAAGGA GAGGGTCCTGGATAGGCTTC
NRGN GGCTCGGTAGGAGGAGTCTT AGCACAGTCACATGCACACA
RAB3A GGTGGTGTCATCAGAACGTG GTCCAACGACTCGGACATCT
RAB4B GGTCAGTGACGCGGAGTTAT AGGGTCCAGGTCCTTCTTGT
TUBB2 AGCTCACCCAGCAGATGTTT CATTTGCTCATCCACCTCCT
TUBB4 CTCGAGGCTTCTGACCTTTG CCAGGGTCGGAGATGAAGTA
GATA1 CCAAGCTTCGTGGAACTCTC AGGCGTTGCATAGGTAGTGG
PPIA CTCGTGCCGTTTTGCAGAC TGCAAACAGCTCAAAGGAGA
Calm2 AGCGAGTCGAGTGGTTGTCT CCGTCGGCATCTACTTCATT
Syn1 CCAGCTCAACAAATCCCAGT GGTGTCAGTCGGAGAAGAGG
Rab3a GGTGGACGTGATCTGTGAGA GAAGGGAAGTGTGGTTCAGC
Rab4b ACTATTGGCGTGGAGTTTGG CACAGAGGATGACCACGATG
Tubb4 GGGGACCTCAACCACCTAGT ATCCTGGCATGAAGAAGTGG
Gata1 GATGGAATCCAGACGAGGAA CTCTCCGCAATTCCCACTAC
Ppia TTGCAGACGCCGCTGTCTCTT TGGAACTTTGTCTGCAAACAGCTCG
GATA1 binding site
Gene Forward (5'->3') Reverse (3'->5')
Calm2 GGGAAGAAGATCCATGGAGAA GGAGAATTCAGCCTGGAAAA
Syn1 ACAGCCTGCATCTCTCCATT ATGTCTGCAAGGGTCCAAAG
Rab3a CTGTCAAGGTCACAGCTTGG GTAGCAGGACCACACACAGG
Rab4b GGCCTGTGGGGTTAGAATTT AAAAATCGGAGCAGAGACGA
Tubb4 CTGCCTCTTCGTCTCTAGCC ACCGCTCAGGCTCACCTT