Figure 2.

MPM and H&E images of DSS-induced colonic injury. A–B, A control mouse colon was imaged showing colonic mucosa with uniform, homogenous crypts. C–D, High magnification images of panels A and B show columnar cells lining the surface epithelium (arrows), crypts (arrowheads and insets), and connective tissue (red in MPM) of the lamina propria. E–F, The colon from a mouse exposed to DSS for 7 days is shown at low magnification with a region of non-ulcerated colonic mucosa (a) and an ulcerated area with crypt loss and exposed connective tissue of the lamina propria (b). G–H, High magnification images of panels E and F show atrophic crypts (arrows and insets) in the non-ulcerated area (a) and exposed lamina propria with inflammatory cells (arrowheads) in the ulcerated area (b). I–J, A colon from a mouse given DSS for 7 days followed by 7 days of plain drinking water shows morphological features of resolving colitis at low magnification including a region with aberrant crypts (a) and an area of crypt loss associated with increased inflammatory cells and connective tissue (b). K–L, High magnification images show enlarged regenerative crypts (arrows and insets) from area ‘a’ in panels I and J and an increased number of mononuclear inflammatory cells (arrowheads) and connective tissue (red in MPM).

(MPM total magnifications: A, E, I = 48X, C, G, K = 300X, insets = 750X. H&E total magnifications: B, F, J = 40X, D, H, L = 200X, insets = 500X).