Figure 3.

Images of AOM-induced colonic neoplasia generated by MPM and H&E. A–D, Colonic mucosa from a saline-administered control mouse at low (A–B) and high (C–D) magnifications showing uniformly spaced homogenous crypts and a lymphoid follicle (arrows). Insets in panels C and D show a normal crypt at higher magnification. E–F, Images of an ACF are shown (dotted circle) on a background of polymorphic crypts within the colon one week following the last AOM injection. G–H, High magnification images of panels E and F show a well-defined ACF surrounded by a band of thick connective tissue (red in MPM). Insets show an ACF lined by cells with low-grade dysplasia defined by elongated and crowded nuclei. I–J, Images show a microadenoma three weeks following the last AOM injection (dotted circle), on a background of polymorphic appearing colonic mucosa. K–L, High magnification images of panels I and J show closely packed aberrant crypts with high-grade dysplasia defined by nuclear stratification and loss of goblet cells (insets) and little intervening connective tissue. M–N, Images show a macroadenoma (arrows) seven weeks following the last AOM injection. O–P, High magnification images of panels M and N show fused glands within the macroadenoma (arrows) and surrounding areas containing aberrant crypts (arrowheads). Insets show cells with high-grade dysplasia lining the adenomatous glands.

(MPM total magnifications: A, E, I, M = 48X, C, G, K, O = 300X, insets = 750X. H&E total magnifications: B, F, J, N = 40X, D, H, L, P = 200X, insets = 400X).