Figure 4.

Imaging of small intestinal lesions from human subjects by MPM and H&E. A–B, Images of the normal duodenum from a control subject show villi (arrows) lining the mucosal surface at low magnification. C–D, High magnification images of panels A and B show enterocytes (green in MPM) and scattered goblet cells (arrows) lining the villi. The core of the villus (a) shows mononuclear cells (mixture of blue and green cells in MPM), recognized mainly as lymphocytes by H&E. E–F, Duodenal biopsy from a subject clinically diagnosed with celiac sprue shows flattened mucosal surface with villus shortening (arrows) and preserved crypts (arrowheads). G–H, High magnification images of panels E and F show the flattened mucosal surface lined by simple columnar cells (arrows) and crypts with goblet cells (arrowheads). The lamina propria (a) has increased inflammatory cell infiltrate (mixture of blue and green cells in MPM), mainly identified as lymphocytes, plasma cells and eosinophils by H&E. I–L, The normal terminal ileum from a control subject shows villi (arrows) at low (I–J) and high (K–L) magnifications. M–P, Ileal biopsy from a subject with Crohn’s disease shows morphological features of chronic active ileitis. Low magnification images (M–N) show blunted villi (arrows), focal ulcerations (arrowheads) and inflammatory infiltrate (a). High magnification images (O–P) show focal ulcerations (arrowheads) and inflammatory infiltrate (a) (identified as neutrophils and lymphocytes by H&E).

(MPM total magnifications: A, E, I, M = 48X; C, G, K, O = 300X. H&E total magnifications: B, F, J, N = 40X ; D, H, L, P = 200X)