Figure 5.

Imaging of colonic lesions from human subjects by MPM and H&E. A–D, Image of normal colonic mucosa from a control subject shows low (A–B) and high (C–D) magnification images of the surface epithelium (arrows) and crypts (arrowheads) with mononuclear cells in the lamina propria recognized mainly as lymphocytes by H&E. E–H, Distal colonic biopsy from a subject with ulcerative colitis shows morphological features of chronic active colitis. Low magnification images (E–F) show irregular mucosal surface due to ulceration/pseudopolyps (arrows), reduced numbers of crypts and increased cellularity in the lamina propria (a). The high magnification images (G–H) show a pseudopolyp lined by columnar epithelium (arrow) with complete loss of crypts and marked mononuclear inflammatory cell infiltrate in the lamina propria (a), identified as lymphocytes and plasma cells on H&E. I–L, Images from a tubular adenoma show a smooth polypoid surface (arrows) with adenomatous glands (arrowheads) at low magnification in panels I–J and high magnification images in panels K–L show an adenomatous gland with low-grade dysplasia (arrows and insets). M-P, A well-differentiated adenocarcinoma of the colon is shown at low magnification in panels M–N with complex (back-to-back) glandular arrangement and shown at high magnification in panels O–P revealing crowded glands lined by dysplastic cells (insets).

(MPM total magnifications: A, E, I, M = 48X; C, G, K, O = 300X. H&E total magnifications: B, F, J, N = 40X, D, H, L, P = 200X).