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. Author manuscript; available in PMC: 2013 Jun 1.
Published in final edited form as: Kidney Int. 2012 Aug 1;82(12):1271–1283. doi: 10.1038/ki.2012.261

Figure 4. Cisplatin-induced autophagy is inhibited in renal proximal tubules in PTAtg7-KO mice.

Figure 4

Figure 4

Wild-type and PT-Atg7-KO mice were injected with 25mg/kg cisplatin or saline as control. (A) After the indicated time, kidneys were harvested to collect cortical tissues for immunoblot analysis of Atg7, LC3, Atg5 (Atg12 conjugated), p62, and β-actin. (B) Three days after injection, kidneys were collected for immunofluorescence staining of LC3, FITC-labeled PHA, and Hoechst33342 (×630). Representative images of cisplatin-treated wild-type and PT-Atg7-KO groups were shown. Arrows point to LC3 dots (autophagosomes) and inset shows LC3 dots at higher magnifications. (C) Quantification of LC3 dots in individual proximal tubule of cisplatin-treated wild-type and PT-Atg7-KO groups. Data are expressed as mean ± SD. * P < 0.05, significantly different from the wild-type group. (D) After 4 days of treatment, kidneys were collected for immunohistochemical staining of p62 (×200). The selected areas were shown at high magnifications.