Figure 1.

Derivation of MutuDC lines. (A) Schematic diagram and procedure for the derivation of MutuDC lines (NB. “p” followed by a number designates “passage”). (B) The number of cell divisions during 1 week is shown for eight different cell lines at different passages (n = 3 for passages 5–10, n = 5 for passage >15), based on trypan blue counts of MutuDC line cultures starting at 5 × 10⁴ cells per 0.5 mL per cm². (C) The growth of one representative MutuDC line as a function of cell density is shown, measured by thymidine incorporation (n = 3) by seeding MutuDC line cells in flat-bottom 96-well plates at the indicated densities and adding 0.5 μCi of 3H-thymidine per well for 15 h. Data are presented as mean ± SD.