Figure 5.

MutuDC lines express TLR3 and lack TLR7, a characteristic of CD8α⁺ cDC. Gene expression was analyzed by Nanostring technology for TLR3 and TLR4 (A) (n = 3 for splenic cDC subsets and n = 13 for MutuDC lines) and by quantitative RT-PCR for TLR7 and TLR9 (B) (n = 2 per splenic cDC subsets and n = 3 for MutuDC lines). Data are presented as mean ± SD. p-values were obtained by One-way ANOVA with Bonferroni post-test comparing all three DC types for each TLR individually (NB. The scale is logarithmic).