Figure 9.

Genetically modified MutuDC lines. (A) One representative example of lentiviral transduction of MutuDC lines is shown, with H-2K^d over-expression and efficient induction of H-2K^d-restricted antigen presentation. H-2K^d-positive or control (untransduced) MutuDC lines were loaded with titrating amounts of the PbCs peptide as indicated and co-cultured with the relevant cognate T1 TCR-Tg CD8 T cells. (B,C) Representative genetically modified MutuDC lines are shown, deficient for Tlr3 and Tlr9. WT (n = 3), Tlr3^−/− (n = 4) or Tlr9^−/− (n = 3) MutuDC lines were stimulated with PolyIC or CpG and analyzed after 18 h by FACS for the activation markers CD40 and CD80. (B) Similarly, the MutuDC lines were stimulated with different combinations of CpG, PolyIC, IFNγ, and aCD40 and analyzed after 18 h by ELISA on supernatants for IL-12 p40 and p70 secretion (n = 1) (C).