Figure A4.

Transfection and electroporation of MutuDC lines. MutuDC line transfectability was tested using classical approaches such as calcium phosphate (CaPi) (A,B), JetPEI (C) or electroporation (D). The DsRedExpress (A–C) or pmaxGFP vectors (D) were used as test examples. CaPi transfection efficiency (A) and consequent upregulation of activation markers (B) 24 h post-transfection measured by flow cytometry (filled gray: negative control, open gray: mock transfection, open black: DsRedExpress transfected cells). (C) Transfection using JetPEI, with much weaker reporter gene expression as compared to CaPi in (A). Electroporation efficiency and cell survival (D) at 24 h using several Amaxa electroporation programs with endotoxin free plasmid or electroporation solutions alone (Solution L and Solution V). In both electroporation and transfection (whether CaPi or JetPEI), all positive cells died within 72 h (data not shown).